Rajiv Gandhi Centre for Aquaculture
(A Society under Marine Products Export Development Authority,
Ministry of Commerce & Industry, Govt. of India)

Technology Development Centre for Sustainable Aquaculture

Central Aquaculture Genetics Laboratory
(Accredited by NABL as per ISO/IEC 17025:2005)

The Central Aquaculture Genetics Laboratory of Rajiv Gandhi Centre for Aquaculture (RGCA), R & D arm of Marine Products Export Development Authority (MPEDA), Ministry of Commerce & Industry, Govt. of India. becomes the First Aquaculture Genetics Laboratory of the country accredited by NABL (National Accreditation Board For Testing & Calibration Laboratories) as per ISO/IEC 17025: 2005. The CAGL thus gets the distinction of being the first Aquaculture Laboratory in the country to obtain NABL recognition which reassured the quality research work going on at the CAGL. 

The Facility:

CAGL is extremely well equipped for contemporary research on molecular & cellular biology and genetics, The CAGL is supplimented with modern equipment and has a dedicated section for conducting molecular genetic studies. The tissue extraction area has equipment such as cooling microcentrifuge, electronic balance, autoclave, automatic water bath system & deep freezer etc., while the PCR area is equipped with thermocycler & laminar flow chamber and post PCR area with electrophoresis system, gel doc system, -80°C deep freezer, spectrophotometer and biophotometer. The CAGL also has a genetic analyzer (ABI 3500) to perform qualitative and quantitative genetic study. 


CAGL plays a key role in selective breeding programmes of shrimp, scampi and tilapia presently being implemented at RGCA. The lab supports to resolve culture related issues of these organisms through implementation of effective breeding strategies and thus helps in enhancing their production and survival rates. Currently the lab is actively involved in population genetics study for targeted species to determine the founder population for selective breeding programmes. CAGL has published population genetics research work in black tiger shrimp of India in reputed International journal ‘GENE’. As the accuracy of species identification is important for any culture activity the lab is conducting research on molecular identification of species with multiple molecular markers. The CAGL has identified the mangrove mud crab species using multiple molecular markers and reported for the first time from India that only two mud crab species of genus Scylla exist in our coastal waters. The lab has many International publications and had submitted more than 60 unique sequence at NCBI GenBank.
  



View of the Central Aquaculture Genetics Laboratory(CAGL).
Gel Documentation at CAGL
Post PCR area at CAGL
DNA Sequencing at CAGL


Present Status & Functions: 

CAGL is conducting the population genetics study for different ongoing projects to find out the suitable founder populations utilizing advanced molecular genetics markers such as microsatellites, mitochondrial-DNA sequencing etc. Genetics lab is involved in evaluating the inbreeding and keeping the targeted gene in breeding stocks utilizing genetics data and constructing pedigree for families. Correct identification of the species is important for successful selective breeding programme. Genetics lab is conducting genetic divergence study using various molecular markers (viz., RAPD, PCR-RFLP, Gene sequencing etc) to resolve taxonomic ambiguity of species and assisting project personnel at hatchery for individual and family identification using PIT and VIE tags.Also involving in conducting selective breeding programmes of the species of respective projects.

Publications: 

Research papers in International journals:
  1. Population genetic structure of Penaeus monodon, in relation to monsoon current patterns in Southwest, East and Andaman coastal waters of India. Authors: Anup Mandal, Divya Rao, Deepa Karuppaiah, Achamveetil Gopalakrishnan, Jayagopal Pozhoth, Yohannan Chellamma Thampi Samraj and Roger W. Doyle. GENE, vol.491, 149-157, 2012.
  2. Molecular Markers Reveal Only Two Mud Crab Species of Genus Scylla (Brachyura: Portunidae) in Indian Coastal Waters. Authors: Anup Mandal, Mathews Varkey, Sobha P. Sobhanan, Anjali K. Mani, A. Gopalakrishnan, Ganesh Kumaran, Arulraj Sethuramalingam, Pandiarajan Srinivasan and Y. C. Thampi Samraj. Biochemical Genetics, vol. 52 (3/4), April 2014.         This is the first report from India resolving the taxonomic ambiguity of commonly available Indian mud crabs using a combination of several molecular markers along with traditional taxonomy. The importance of this study is immense because proper identification plays pivotal role in breeding and management of the particular 
  3. Identification of Indian mangrove mud crab Genus Scylla spp (Brachyura: Portunidae) using RAPD and PCR-RFLP markers. Authors: Anup Mandal, Mathews Varkey, Anjali K. Mani, Sobha P. Sobhanan and Y. C. Thampi Samraj. Journal of Shellfish Research, Vol. 33, No. 2, 1–8, August 2014.
NCBI GenBank:

The CAGL has published 92 unique sequences for different species, mainly for Indian mangrove mud crabs and groupers. The details are available in the public domain if searched in NCBI website with following GenBank accession numbers. The accessions numbers for mangrove mud crabs species are: AB857337-AB857359; AB861521- AB861522; AB861881-AB861887; KC200562-KC200565; KJ607959-KJ607961; & KT921339-KT921350; for groupers species are: KJ607962-KJ607974, KT921351- KT921353 & KT835668-KT835691 and for Temnopleurus toreumaticus is KC262643;
 
    

The Team

Project Manager :    Dr. Anup Mandal
Asst. Technical Managers :    Shri. E. Ananda Jothi
:    Shri. L. Ruban
:    Smt. Sobha P.S.
:    Ms. Anjali K.M.
Technician :    Shri. K. Ajeeth


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